Hotspot

rps4

Genome · 52383-53009 · 627 bp

Back to Vandenboschia

Pi 0.00159

MarkerSeek score 59.70

Primer available yes

Label rps4

Feature Metadata

label_name: rps4
feature_type: gene
region: Genome
length_bp: 627
variable_sites: 1
pi: 0.00159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	TAAGAAGAGATCCCTCGGCT	ACAGGTAAACGGCTCATTCA	50.0	45.0	57.2	57.1	800 bp	1.0	67.2
rps4_p2	AAGAAGAGATCCCTCGGCTG	ACAGGTAAACGGCTCATTCA	55.0	45.0	58.9	57.1	799 bp	1.0	66.2
rps4_p3	GAAGAGATCCCTCGGCTGAA	ACAGGTAAACGGCTCATTCA	55.0	45.0	58.9	57.1	797 bp	1.0	66.2
rps4_p4	TAAGAAGAGATCCCTCGGCT	CAGGTAAACGGCTCATTCAGA	50.0	47.6	57.2	58.0	799 bp	1.0	65.8
rps4_p5	TAAGAAGAGATCCCTCGGCT	AACAGGTAAACGGCTCATTCA	50.0	42.9	57.2	57.9	801 bp	1.0	65.0