Hotspot

rps18

Genome · 72462-72689 · 228 bp

Back to Vandenboschia

Pi 0

MarkerSeek score 32.60

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: Genome
length_bp: 228
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	TCAGTAATTCTGATAGTTCTGAGA	TCTAATTCACTCTTCTGGTTT	33.3	33.3	54.6	52.1	307 bp	1.0	40.0
rps18_p2	TCAGTAATTCTGATAGTTCTGAGA	TTCTAATTCACTCTTCTGGTT	33.3	33.3	54.6	52.1	308 bp	1.0	40.0
rps18_p3	TCAGTAATTCTGATAGTTCTGAGA	TCTAATTCACTCTTCTGGTTTT	33.3	31.8	54.6	53.0	307 bp	1.0	40.0
rps18_p4	TCAGTAATTCTGATAGTTCTGAGA	TTCTAATTCACTCTTCTGGTTT	33.3	31.8	54.6	53.0	308 bp	1.0	40.0
rps18_p5	TCAGTAATTCTGATAGTTCTGAGA	TTCTAATTCACTCTTCTGGTTTT	33.3	30.4	54.6	53.9	308 bp	1.0	40.0