Hotspot

psbC

Genome · 39684-41105 · 1422 bp

Back to Vandenboschia

Pi 0.00141

MarkerSeek score 59.60

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: Genome
length_bp: 1422
variable_sites: 2
pi: 0.00141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TGGCAAGGGCATTCTTACAA	TTGGATGGCAGCTCAAGATC	45.0	50.0	57.7	58.0	1489 bp	1.0	77.3
psbC_p2	TGGCAAGGGCATTCTTACAA	CTTGGATGGCAGCTCAAGAT	45.0	50.0	57.7	57.9	1490 bp	1.0	77.3
psbC_p3	ATGGCAAGGGCATTCTTACA	TTGGATGGCAGCTCAAGATC	45.0	50.0	57.5	58.0	1490 bp	1.0	75.5
psbC_p4	ATGGCAAGGGCATTCTTACA	CTTGGATGGCAGCTCAAGAT	45.0	50.0	57.5	57.9	1491 bp	1.0	75.4
psbC_p5	TGGCAAGGGCATTCTTACAA	GCGGAAGATCCTGAGTTTGA	45.0	50.0	57.7	57.6	1554 bp	1.0	74.6