Hotspot

matK

Genome · 8024-9529 · 1506 bp

Back to Vandenboschia

Pi 0.00133

MarkerSeek score 59.50

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: Genome
length_bp: 1506
variable_sites: 2
pi: 0.00133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TGGAGAAATAATCTACAGCCT	TGGATTCACAAGACCTACGG	38.1	50.0	53.7	56.9	1631 bp	1.0	40.1
matK_p2	TGGAGAAATAATCTACAGCCTACT	TGGATTCACAAGACCTACGG	37.5	50.0	56.7	56.9	1631 bp	1.0	40.1
matK_p3	TGGAGAAATAATCTACAGCCT	GGATTCACAAGACCTACGGA	38.1	50.0	53.7	56.7	1630 bp	1.0	40.1
matK_p4	TGGAGAAATAATCTACAGCCTACT	GGATTCACAAGACCTACGGA	37.5	50.0	56.7	56.7	1630 bp	1.0	40.1
matK_p5	TGGAGAAATAATCTACAGCCTACTT	TGGATTCACAAGACCTACGG	36.0	50.0	57.3	56.9	1631 bp	1.0	40.1