Hotspot

chlN

Genome · 126527-127921 · 1395 bp

Back to Vandenboschia

Pi 0.000717

MarkerSeek score 59.20

Primer available yes

Label chlN

Feature Metadata

label_name: chlN
feature_type: gene
region: Genome
length_bp: 1395
variable_sites: 1
pi: 0.000717
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN_p1	TCAATCAGTTGTCAATTCTTGGT	ACCCAAAATGAACAGGACGT	34.8	45.0	56.6	57.6	1532 bp	1.0	42.2
chlN_p2	TCAATCAGTTGTCAATTCTTGGT	AACAGGACGTTCTGCTTGAT	34.8	45.0	56.6	57.4	1522 bp	1.0	40.7
chlN_p3	TCAATCAGTTGTCAATTCTTGGT	ACAGGACGTTCTGCTTGATT	34.8	45.0	56.6	57.4	1521 bp	1.0	40.7
chlN_p4	CAATCAGTTGTCAATTCTTGGT	ACCCAAAATGAACAGGACGT	36.4	45.0	55.2	57.6	1531 bp	1.0	40.1
chlN_p5	TCCATCAATCAGTTGTCAATTCT	ACCCAAAATGAACAGGACGT	34.8	45.0	56.2	57.6	1536 bp	1.0	40.1