Hotspot

atpB

Genome · 58696-60177 · 1482 bp

Back to Vandenboschia

Pi 0.00135
MarkerSeek score 59.50
Primer available yes
Label atpB

Feature Metadata

label_name
atpB
feature_type
gene
region
Genome
length_bp
1482
variable_sites
2
pi
0.00135
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB_p1 TTGGGAGCCATTACACGAAG TACCATGGACGGATTAGCGA 50.0 50.0 57.9 58.3 1600 bp 1.0 76.8
atpB_p2 AACAATTCGATTGGGAGCCA TACCATGGACGGATTAGCGA 45.0 50.0 57.8 58.3 1610 bp 1.0 75.9
atpB_p3 CGCATGATTGGGCAATATGC TACCATGGACGGATTAGCGA 50.0 50.0 58.3 58.3 1682 bp 1.0 75.3
atpB_p4 TTGGGAGCCATTACACGAAG TTACCATGGACGGATTAGCG 50.0 50.0 57.9 57.5 1601 bp 1.0 75.1
atpB_p5 GATTGGGAGCCATTACACGA TACCATGGACGGATTAGCGA 50.0 50.0 57.7 58.3 1602 bp 1.0 75.1