Hotspot

rps18-rpl20

Genome · 72690-72913 · 224 bp

Back to Vandenboschia

Pi 0.0312

MarkerSeek score 70.50

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: Genome
length_bp: 224
variable_sites: 7
pi: 0.0312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	ATGTGTCAATAGGTTGGCCG	CAGACACTTCTTGTTTTTCCAGA	50.0	39.1	58.2	57.2	374 bp	1.0	49.0
rps18-rpl20_p2	AAGGGCTCGTGTTTTAGCTT	CAGACACTTCTTGTTTTTCCAGA	45.0	39.1	57.7	57.2	320 bp	1.0	49.0
rps18-rpl20_p3	AGGGCTCGTGTTTTAGCTTT	CAGACACTTCTTGTTTTTCCAGA	45.0	39.1	57.7	57.2	319 bp	1.0	49.0
rps18-rpl20_p4	AGGGCTCGTGTTTTAGCTTT	TCAGACACTTCTTGTTTTTCCAG	45.0	39.1	57.7	57.2	320 bp	1.0	49.0
rps18-rpl20_p5	ATGTGTCAATAGGTTGGCCG	TCAGACACTTCTTGTTTTTCCAG	50.0	39.1	58.2	57.2	375 bp	1.0	48.9