Hotspot

psbE-petL

Genome · 69901-70801 · 901 bp

Back to Vandenboschia

Pi 0.00222
MarkerSeek score 60.00
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
Genome
length_bp
901
variable_sites
2
pi
0.00222
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AACCACCCTGCAATGAAGAG GTCGAATCTTGTTCAATCCAACA 50.0 39.1 58.1 57.6 1096 bp 1.0 52.6
psbE-petL_p2 TCAGCAAAAGGACGTTCTCC GTCGAATCTTGTTCAATCCAACA 50.0 39.1 58.1 57.6 1024 bp 1.0 52.3
psbE-petL_p3 CCACCCTGCAATGAAGAGAG GTCGAATCTTGTTCAATCCAACA 55.0 39.1 58.3 57.6 1094 bp 1.0 51.3
psbE-petL_p4 TGTCAGCAAAAGGACGTTCT GTCGAATCTTGTTCAATCCAACA 45.0 39.1 57.7 57.6 1026 bp 1.0 50.7
psbE-petL_p5 AACCACCCTGCAATGAAGAG AGTCGAATCTTGTTCAATCCAAC 50.0 39.1 58.1 57.4 1097 bp 1.0 50.6