Hotspot

psaM-ycf12

Genome · 15487-15731 · 245 bp

Back to Vandenboschia

Pi 0

MarkerSeek score 33.80

Primer available yes

Label psaM-ycf12

Feature Metadata

label_name: psaM-ycf12
feature_type: igs
region: Genome
length_bp: 245
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaM-ycf12_p1	ACCGTCCGAAATTGATGTCA	CCCTTTCGAGCTGCTGATAG	45.0	55.0	57.5	58.1	358 bp	1.0	75.2
psaM-ycf12_p2	TGACCGTCCGAAATTGATGT	CCCTTTCGAGCTGCTGATAG	45.0	55.0	57.5	58.1	360 bp	1.0	75.2
psaM-ycf12_p3	ACCGTCCGAAATTGATGTCA	AGATTTCCCTTTCGAGCTGC	45.0	50.0	57.5	58.3	364 bp	1.0	74.2
psaM-ycf12_p4	ACCGTCCGAAATTGATGTCA	GATTTCCCTTTCGAGCTGCT	45.0	50.0	57.5	58.3	363 bp	1.0	74.2
psaM-ycf12_p5	TGACCGTCCGAAATTGATGT	GATTTCCCTTTCGAGCTGCT	45.0	50.0	57.5	58.3	365 bp	1.0	74.2