Hotspot

ndhD-psaC

SSC · 115717-115844 · 128 bp

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Pi 0.165
MarkerSeek score 74.80
Primer available yes
Label ndhD-psaC

Feature Metadata

label_name
ndhD-psaC
feature_type
igs
region
SSC
length_bp
128
variable_sites
21
pi
0.165
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-psaC_p1 TTTGCCCACTGTACATTGCT GATACCCTGGGACGGATGTA 45.0 55.0 58.0 58.0 666-667 bp 1.0 84.0
ndhD-psaC_p2 AACCGATACCCAGAACTCCA GATACCCTGGGACGGATGTA 50.0 55.0 58.1 58.0 849-850 bp 1.0 83.1
ndhD-psaC_p3 ACCGATACCCAGAACTCCAA GATACCCTGGGACGGATGTA 50.0 55.0 58.1 58.0 848-849 bp 1.0 83.1
ndhD-psaC_p4 CCCCCTGCTGTGTACAAAAT GATACCCTGGGACGGATGTA 50.0 55.0 58.1 58.0 811-812 bp 1.0 83.1
ndhD-psaC_p5 ATAATGCGCTTCTCCATGGG GATACCCTGGGACGGATGTA 50.0 55.0 58.1 58.0 1019-1020 bp 1.0 82.5