Hotspot

petN-psbM

LSC · 29327-30325 · 999 bp

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Pi 0.0609

MarkerSeek score 79.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 999
variable_sites: 59
pi: 0.0609
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	ACGTAAAAACAGTCAGTCAA	50.0	35.0	58.0	53.0	1042-1059 bp	1.0	47.0
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	ACGTAAAAACAGTCAGTCAA	50.0	35.0	58.0	53.0	1040-1057 bp	1.0	47.0
petN-psbM_p3	CGTAGTGTGGGGAAGAAGTG	ACGTAAAAACAGTCAGTCAA	55.0	35.0	57.9	53.0	1039-1056 bp	1.0	46.8
petN-psbM_p4	ACTCGTAGTGTGGGGAAGAA	ACGTAAAAACAGTCAGTCAAA	50.0	33.3	58.0	53.9	1042-1059 bp	1.0	46.8
petN-psbM_p5	TCGTAGTGTGGGGAAGAAGT	ACGTAAAAACAGTCAGTCAAA	50.0	33.3	58.0	53.9	1040-1057 bp	1.0	46.8