Hotspot

trnC-GCA-petN

LSC · 28375-29236 · 862 bp

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Pi 0.0387
MarkerSeek score 76.80
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
862
variable_sites
32
pi
0.0387
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG ACTTCTTCCCCACACTACGA 50.0 50.0 58.1 58.0 950-972 bp 1.0 84.9
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG TTCTTCCCCACACTACGAGT 50.0 50.0 58.1 58.0 948-970 bp 1.0 84.9
trnC-GCA-petN_p3 TTTTGGATCATTTTGGCGGC ACTTCTTCCCCACACTACGA 45.0 50.0 57.9 58.0 1006-1028 bp 1.0 84.5
trnC-GCA-petN_p4 TTTTGGATCATTTTGGCGGC TTCTTCCCCACACTACGAGT 45.0 50.0 57.9 58.0 1004-1026 bp 1.0 84.5
trnC-GCA-petN_p5 TTTCCCCAGTTCAAATCCGG CACTTCTTCCCCACACTACG 50.0 55.0 58.1 57.9 951-973 bp 1.0 84.3