Hotspot

rpoB-trnC-GCA

LSC · 27086-28303 · 1218 bp

Back to Vandellia

Pi 0.0358
MarkerSeek score 74.60
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1218
variable_sites
43
pi
0.0358
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 AGTTCTTCTGTCAAACCCTGA CCGGATTTGAACTGGGGAAA 42.9 50.0 56.8 58.1 1360-1378 bp 1.0 65.2
rpoB-trnC-GCA_p2 TCAAACCCTGATCAATGAACCT CCGGATTTGAACTGGGGAAA 40.9 50.0 57.6 58.1 1350-1368 bp 1.0 63.8
rpoB-trnC-GCA_p3 TCTTCTGTCAAACCCTGATCA CCGGATTTGAACTGGGGAAA 42.9 50.0 56.6 58.1 1357-1375 bp 1.0 63.5
rpoB-trnC-GCA_p4 GTCAAACCCTGATCAATGAACC CCGGATTTGAACTGGGGAAA 45.5 50.0 57.6 58.1 1351-1369 bp 1.0 63.4
rpoB-trnC-GCA_p5 AGTTCTTCTGTCAAACCCTGA AAAAGGATTTGCAGTCCCCC 42.9 50.0 56.8 58.4 1342-1360 bp 1.0 63.1