Hotspot

ndhI-rps15

SSC · 117331-117461 · 131 bp

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Pi 0.0373
MarkerSeek score 58.20
Primer available yes
Label ndhI-rps15

Feature Metadata

label_name
ndhI-rps15
feature_type
igs
region
SSC
length_bp
131
variable_sites
19
pi
0.0373
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI-rps15_p1 TGTCCAATGTACCTTGTGGC ATCACAAAGGGGTCTACGAA 50.0 45.0 58.1 56.2 257-286 bp 0.888889 70.1
ndhI-rps15_p2 TGTCCAATGTACCTTGTGGC TCACAAAGGGGTCTACGAAT 50.0 45.0 58.1 56.2 256-285 bp 0.888889 70.1
ndhI-rps15_p3 AAATAAGGAACTGCGTGGCA ATCACAAAGGGGTCTACGAA 45.0 45.0 58.1 56.2 495-517 bp 0.888889 69.0
ndhI-rps15_p4 AAATAAGGAACTGCGTGGCA TCACAAAGGGGTCTACGAAT 45.0 45.0 58.1 56.2 494-516 bp 0.888889 69.0
ndhI-rps15_p5 CCAAAGGTCCGCAAATAGGA TCACAAAGGGGTCTACGAAT 50.0 45.0 57.9 56.2 600-605 bp 0.777778 57.9