Hotspot

trnN-GUU-rpl32

IRb · 111216-112012 · 797 bp

Back to Vanda

Pi 0.0506
MarkerSeek score 84.50
Primer available yes
Label trnN-GUU-rpl32

Feature Metadata

label_name
trnN-GUU-rpl32
feature_type
igs
region
IRb
length_bp
797
variable_sites
108
pi
0.0506
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rpl32_p1 AGTCAGTTAACAGCCAACCG GCTTTTACTGCCTCTAAAGATCC 50.0 43.5 58.1 57.3 889-1000 bp 1.0 61.1
trnN-GUU-rpl32_p2 ACGACCAGTCAGTTAACAGC GCTTTTACTGCCTCTAAAGATCC 50.0 43.5 57.8 57.3 895-1006 bp 1.0 60.9
trnN-GUU-rpl32_p3 AGGATTCGAACCTACGACCA GCTTTTACTGCCTCTAAAGATCC 50.0 43.5 58.2 57.3 908-1019 bp 1.0 60.8
trnN-GUU-rpl32_p4 AGTCAGTTAACAGCCAACCG AGAAAAAGCTTTTACTGCCTCT 50.0 36.4 58.1 56.2 896-1007 bp 1.0 60.5
trnN-GUU-rpl32_p5 GGATTCGAACCTACGACCAG GCTTTTACTGCCTCTAAAGATCC 55.0 43.5 57.8 57.3 907-1018 bp 1.0 60.4