Hotspot

psbL-psbF

LSC · 65530-65551 · 22 bp

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Pi 0.0202
MarkerSeek score 56.00
Primer available yes
Label psbL-psbF

Feature Metadata

label_name
psbL-psbF
feature_type
igs
region
LSC
length_bp
22
variable_sites
2
pi
0.0202
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbL-psbF_p1 CATTCGGGTTTGATTGTGTCA GGACTGGCTGTACCTACTGT 42.9 55.0 57.1 58.4 110 bp 1.0 62.6
psbL-psbF_p2 TCATTCGGGTTTGATTGTGTC GGACTGGCTGTACCTACTGT 42.9 55.0 56.8 58.4 111 bp 1.0 60.4
psbL-psbF_p3 TGTTCATTCGGGTTTGATTGTG GGACTGGCTGTACCTACTGT 40.9 55.0 57.8 58.4 114 bp 1.0 60.0
psbL-psbF_p4 GTTCATTCGGGTTTGATTGTGT GGACTGGCTGTACCTACTGT 40.9 55.0 57.8 58.4 113 bp 1.0 60.0
psbL-psbF_p5 CATTCGGGTTTGATTGTGTCAT GGACTGGCTGTACCTACTGT 40.9 55.0 57.6 58.4 110 bp 1.0 58.4