Hotspot

ndhC-trnV-UAC

LSC · 51215-51923 · 709 bp

Back to Vanda

Pi 0.0335
MarkerSeek score 65.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
709
variable_sites
55
pi
0.0335
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.94
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 ATCATATTCGTGAAATAGAAACA AGAAGGTCTACGGTTCGAGT 26.1 50.0 52.0 57.8 666-1031 bp 0.777778 40.2
ndhC-trnV-UAC_p2 TCATATTCGTGAAATAGAAACAT AGAAGGTCTACGGTTCGAGT 26.1 50.0 52.0 57.8 665-1030 bp 0.777778 40.2
ndhC-trnV-UAC_p3 ATCATATTCGTGAAATAGAAACA GAAGGTCTACGGTTCGAGTC 26.1 55.0 52.0 57.5 665-1030 bp 0.777778 40.2
ndhC-trnV-UAC_p4 TCATATTCGTGAAATAGAAACAT GAAGGTCTACGGTTCGAGTC 26.1 55.0 52.0 57.5 664-1029 bp 0.777778 40.2
ndhC-trnV-UAC_p5 ATCATATTCGTGAAATAGAAACAT AGAAGGTCTACGGTTCGAGT 25.0 50.0 52.7 57.8 666-1031 bp 0.777778 40.2