Hotspot

psbC-trnS-UGA

LSC · 36812-36956 · 145 bp

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Pi 0.0195
MarkerSeek score 59.40
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
145
variable_sites
12
pi
0.0195
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 CCTGTTCTTTCCATGACCCC GAGAGATGGCTGAGTGGTTG 55.0 55.0 58.2 58.0 256-284 bp 1.0 85.7
psbC-trnS-UGA_p2 CCTGTTCTTTCCATGACCCC ATAGCCCCGGTCTTGAAAAC 55.0 50.0 58.2 57.9 236-264 bp 1.0 85.5
psbC-trnS-UGA_p3 TTCCATGACCCCTCTTAGCT GAGAGATGGCTGAGTGGTTG 50.0 55.0 57.7 58.0 248-276 bp 1.0 85.1
psbC-trnS-UGA_p4 AGCGGGGTTTGAAAAAGGAA GAGAGATGGCTGAGTGGTTG 45.0 55.0 58.2 58.0 293-321 bp 1.0 84.4
psbC-trnS-UGA_p5 TTGTGGGACATTTGTGGCAT GAGAGATGGCTGAGTGGTTG 45.0 55.0 58.3 58.0 336-364 bp 1.0 83.1