Hotspot

petN-psbM

LSC · 29862-30672 · 811 bp

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Pi 0.0197
MarkerSeek score 59.60
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
811
variable_sites
48
pi
0.0197
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 GGGGCATCATTCACATGGAT ACGTCAAAACAGTCAGCCAA 50.0 45.0 58.0 58.3 925-1011 bp 1.0 86.0
petN-psbM_p2 GGGGCATCATTCACATGGAT ATTGCTACTGCGCTGTTCAT 50.0 45.0 58.0 58.3 977-1063 bp 1.0 85.8
petN-psbM_p3 TCCTGTTTTGACTCTGCACC ACGTCAAAACAGTCAGCCAA 50.0 45.0 58.0 58.3 1010-1100 bp 1.0 85.6
petN-psbM_p4 GGGGCATCATTCACATGGAT CGTTTATTGCTACTGCGCTG 50.0 50.0 58.0 57.9 982-1047 bp 0.888889 82.6
petN-psbM_p5 TCCTGTTTTGACTCTGCACC CGTTTATTGCTACTGCGCTG 50.0 50.0 58.0 57.9 1067-1136 bp 0.888889 82.3