Hotspot

rpoB-trnC-GCA

LSC · 27305-28924 · 1620 bp

Back to Vanda

Pi 0.0129
MarkerSeek score 57.80
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1620
variable_sites
54
pi
0.0129
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.94
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 GTGGACATTCCCTCATTTCCA CCGGGGATAAAGGATTTGCA 47.6 50.0 57.9 57.9 1468-1703 bp 1.0 77.4
rpoB-trnC-GCA_p2 TGTGGACATTCCCTCATTTCC CCGGGGATAAAGGATTTGCA 47.6 50.0 57.9 57.9 1469-1704 bp 1.0 77.4
rpoB-trnC-GCA_p3 GTGGACATTCCCTCATTTCCA ACCGGGGATAAAGGATTTGC 47.6 50.0 57.9 57.9 1469-1704 bp 1.0 77.4
rpoB-trnC-GCA_p4 TGTGGACATTCCCTCATTTCC ACCGGGGATAAAGGATTTGC 47.6 50.0 57.9 57.9 1470-1705 bp 1.0 77.4
rpoB-trnC-GCA_p5 GTGGACATTCCCTCATTTCCA TCGAACCGGGGATAAAGGAT 47.6 50.0 57.9 58.2 1473-1708 bp 1.0 76.3