Hotspot

rps16-trnQ-UUG

LSC · 6092-6962 · 871 bp

Back to Vanda

Pi 0.0203
MarkerSeek score 64.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
871
variable_sites
58
pi
0.0203
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 ATCCACAGCCAATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 952-1039 bp 1.0 86.4
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 929-1016 bp 1.0 86.0
rps16-trnQ-UUG_p3 CCACAGCCAATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 950-1037 bp 1.0 85.7
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 940-1027 bp 1.0 85.4
rps16-trnQ-UUG_p5 ATCCACAGCCAATCATGTCC CTCGGAGGTTCGAATCCTTC 50.0 55.0 57.9 57.8 956-1043 bp 1.0 85.3