Hotspot

matK-trnK-UUU

LSC · 3263-4403 · 1141 bp

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Pi 0.0158
MarkerSeek score 57.30
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
1141
variable_sites
52
pi
0.0158
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 TCCTGAAGGAGTAGCGGATA GGTAGAGTACTCGGCTTTTA 50.0 45.0 57.0 53.9 1207-1280 bp 1.0 47.3
matK-trnK-UUU_p2 TCCTGAAGGAGTAGCGGATA GGTAGAGTACTCGGCTTTTAA 50.0 42.9 57.0 54.8 1207-1280 bp 1.0 47.3
matK-trnK-UUU_p3 TCCTGAAGGAGTAGCGGATAT GGTAGAGTACTCGGCTTTTA 47.6 45.0 57.5 53.9 1207-1280 bp 1.0 47.3
matK-trnK-UUU_p4 TCCTGAAGGAGTAGCGGATAT GGTAGAGTACTCGGCTTTTAA 47.6 42.9 57.5 54.8 1207-1280 bp 1.0 47.3
matK-trnK-UUU_p5 TCCTGAAGGAGTAGCGGAT GGTAGAGTACTCGGCTTTTA 52.6 45.0 57.1 53.9 1207-1280 bp 1.0 47.3