Hotspot

ndhD

SSC · 119886-121388 · 1503 bp

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Pi 0.000665

MarkerSeek score 47.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 1
pi: 0.000665
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TCTCAAACAAAAACTCCAAACGT	TCGAGTATGGGTTTTTCTGTCC	34.8	45.5	57.6	58.1	1649 bp	1.0	40.0
ndhD_p2	TGGTTCTCAAACAAAAACTCCA	TCGAGTATGGGTTTTTCTGTCC	36.4	45.5	56.6	58.1	1653 bp	1.0	40.0
ndhD_p3	TCTCAAACAAAAACTCCAAACGT	TGGGTTTTTCTGTCCAAAGTG	34.8	42.9	57.6	56.8	1642 bp	1.0	40.0
ndhD_p4	TCTCAAACAAAAACTCCAAACGT	GGGTTTTTCTGTCCAAAGTGT	34.8	42.9	57.6	56.8	1641 bp	1.0	40.0
ndhD_p5	TGGTTCTCAAACAAAAACTCCA	TGGGTTTTTCTGTCCAAAGTG	36.4	42.9	56.6	56.8	1646 bp	1.0	40.0