Hotspot

ccsA-ndhD

SSC · 119684-119885 · 202 bp

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Pi 0.0297

MarkerSeek score 60.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 202
variable_sites: 6
pi: 0.0297
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CAATTGTGGCTTCTACGGGA	CCTATCAGTTGACAGGGTAGA	50.0	47.6	57.9	55.9	364 bp	1.0	55.6
ccsA-ndhD_p2	ACTCTGCAATTGTGGCTTCT	CCTATCAGTTGACAGGGTAGA	45.0	47.6	57.7	55.9	370 bp	1.0	54.1
ccsA-ndhD_p3	CAATTGTGGCTTCTACGGGA	CCTATCAGTTGACAGGGTAGAA	50.0	45.5	57.9	56.6	364 bp	1.0	53.6
ccsA-ndhD_p4	TGTGGCTTCTACGGGATTTC	CCTATCAGTTGACAGGGTAGA	50.0	47.6	57.6	55.9	360 bp	1.0	53.3
ccsA-ndhD_p5	CTGCAATTGTGGCTTCTACG	CCTATCAGTTGACAGGGTAGA	50.0	47.6	57.5	55.9	367 bp	1.0	52.2