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psaJ-rpl33

LSC · 68747-69167 · 421 bp

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Pi 0.019

MarkerSeek score 51.20

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 421
variable_sites: 8
pi: 0.019
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACCCGTGACATTTTGTACCC	TACATACGCGCGCTCTTTAA	50.0	45.0	58.1	57.8	1195-1221 bp	1.0	79.2
psaJ-rpl33_p2	CCCGTGACATTTTGTACCCA	TACATACGCGCGCTCTTTAA	50.0	45.0	58.1	57.8	1194-1220 bp	1.0	79.2
psaJ-rpl33_p3	ACAGGATTTGAACCCGTGAC	TACATACGCGCGCTCTTTAA	50.0	45.0	58.1	57.8	1206-1232 bp	1.0	79.1
psaJ-rpl33_p4	CAGGATTTGAACCCGTGACA	TACATACGCGCGCTCTTTAA	50.0	45.0	58.1	57.8	1205-1231 bp	1.0	79.1
psaJ-rpl33_p5	ATGCGTTGACATTCCCCTTT	TACATACGCGCGCTCTTTAA	45.0	45.0	58.1	57.8	681-707 bp	1.0	78.9