Hotspot

trnP-UGG-psaJ

LSC · 68256-68611 · 356 bp

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Pi 0.0201

MarkerSeek score 61.90

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 356
variable_sites: 7
pi: 0.0201
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	AAACCTGCCAAAGACCCAAA	50.0	45.0	58.1	58.1	485 bp	1.0	80.5
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	AAACCTGCCAAAGACCCAAA	50.0	45.0	58.1	58.1	484 bp	1.0	80.5
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	AAACCTGCCAAAGACCCAAA	50.0	45.0	58.1	58.1	496 bp	1.0	80.4
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	AAACCTGCCAAAGACCCAAA	50.0	45.0	58.1	58.1	495 bp	1.0	80.4
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	AAACCTGCCAAAGACCCAAA	50.0	45.0	58.1	58.1	498 bp	1.0	80.4