Hotspot

trnT-GGU-psbD

LSC · 32736-33912 · 1177 bp

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Pi 0.00171

MarkerSeek score 60.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1177
variable_sites: 2
pi: 0.00171
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AAGCGAATGGGCCCTTTTAA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.1	58.2	1341-1351 bp	1.0	78.2
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1331-1341 bp	1.0	77.9
trnT-GGU-psbD_p3	ATGGGCCCTTTTAACTCAGC	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1335-1345 bp	1.0	77.6
trnT-GGU-psbD_p4	AAGCGAATGGGCCCTTTTAA	CAAAAACGAAACGGTCCCTC	45.0	50.0	58.1	57.6	1340-1350 bp	1.0	77.0
trnT-GGU-psbD_p5	AAGCGAATGGGCCCTTTTAA	ATAAGCCGGACCAACCTACA	45.0	50.0	58.1	58.4	1358-1368 bp	1.0	76.8