Hotspot

ycf3

LSC · 50495-52557 · 2063 bp

Back to Vallisneria

Pi 0.481

MarkerSeek score 78.30

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 2063
variable_sites: 797
pi: 0.481
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	AGGAAGGGGGATCTTCATGT	ATCCTCTCCTTCCGGGTTTT	50.0	50.0	57.7	58.3	2949 bp	0.5	55.0
ycf3_p2	ATGTTTGTCAAGACCGACGG	ATCCTCTCCTTCCGGGTTTT	50.0	50.0	58.5	58.3	2933 bp	0.5	53.4
ycf3_p3	AGGAAGGGGGATCTTCATGT	TATCCTCTCCTTCCGGGTTT	50.0	50.0	57.7	57.4	2950 bp	0.5	53.1
ycf3_p4	AGGAAGGGGGATCTTCATGT	TTATCCTCTCCTTCCGGGTT	50.0	50.0	57.7	57.4	2951 bp	0.5	53.1
ycf3_p5	AGGAAGGGGGATCTTCATGT	TTTATCCTCTCCTTCCGGGT	50.0	50.0	57.7	57.4	2952 bp	0.5	53.1