Hotspot

rps3

LSC · 87496-88152 · 657 bp

Back to Vallisneria

Pi 0.18

MarkerSeek score 78.90

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 657
variable_sites: 118
pi: 0.18
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	CGTGCATATCGTGTCATTGC	TGTCTGTTTTCAAAGCCCGA	50.0	45.0	58.0	58.0	1429 bp	0.5	59.6
rps3_p2	TCGCCCAGCTTCTATTTGTC	TGTCTGTTTTCAAAGCCCGA	50.0	45.0	58.0	58.0	1406 bp	0.5	59.4
rps3_p3	CGCCCAGCTTCTATTTGTCT	TGTCTGTTTTCAAAGCCCGA	50.0	45.0	58.0	58.0	1405 bp	0.5	59.4
rps3_p4	CGTGCATATCGTGTCATTGC	GATCAAATTCGCGGACGTTC	50.0	50.0	58.0	57.9	1400 bp	0.5	59.3
rps3_p5	TTTGCCGTTGCTCTTACTGT	TGTCTGTTTTCAAAGCCCGA	45.0	45.0	58.0	58.0	1495 bp	0.5	59.3