Hotspot

trnP-UGG-rps18

LSC · 71337-72797 · 1461 bp

Back to Vallisneria

Pi 0.235

MarkerSeek score 83.40

Primer available yes

Label trnP-UGG-rps18

Feature Metadata

label_name: trnP-UGG-rps18
feature_type: igs
region: LSC
length_bp: 1461
variable_sites: 316
pi: 0.235
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-rps18_p1	CAATACAGGCGTGGTGATCA	CGAGTATTTCTAGGACCGCG	50.0	55.0	58.0	58.0	2210 bp	0.5	59.5
trnP-UGG-rps18_p2	ACAATACAGGCGTGGTGATC	CGAGTATTTCTAGGACCGCG	50.0	55.0	58.0	58.0	2211 bp	0.5	59.5
trnP-UGG-rps18_p3	CAATACAGGCGTGGTGATCA	AGTCCAAGAAGAAGGAGGCT	50.0	50.0	58.0	58.0	2313 bp	0.5	59.5
trnP-UGG-rps18_p4	ACAATACAGGCGTGGTGATC	AGTCCAAGAAGAAGGAGGCT	50.0	50.0	58.0	58.0	2314 bp	0.5	59.5
trnP-UGG-rps18_p5	GTTTTGGAGACCCGTGTTCT	CGAGTATTTCTAGGACCGCG	50.0	55.0	58.0	58.0	2043 bp	0.5	59.4