Hotspot

trnW-CCA-trnP-UGG

LSC · 71051-71262 · 212 bp

Back to Vallisneria

Pi 0.236

MarkerSeek score 81.30

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 212
variable_sites: 46
pi: 0.236
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GTTTTGGAGACCCGTGTTCT	GGTAGCGTGTTTGTTTTGGG	50.0	50.0	58.0	57.9	309-396 bp	1.0	90.5
trnW-CCA-trnP-UGG_p2	GTTTTGGAGACCCGTGTTCT	CCGGTAGCGTGTTTGTTTTG	50.0	50.0	58.0	58.3	311-398 bp	1.0	89.5
trnW-CCA-trnP-UGG_p3	GTTTTGGAGACCCGTGTTCT	CGGTAGCGTGTTTGTTTTGG	50.0	50.0	58.0	58.3	310-397 bp	1.0	89.5
trnW-CCA-trnP-UGG_p4	ACCCGTGTTCTACCAAACTG	GGTAGCGTGTTTGTTTTGGG	50.0	50.0	57.8	57.9	300-387 bp	1.0	88.9
trnW-CCA-trnP-UGG_p5	ATTTGAACCTACGGCATCGG	GGTAGCGTGTTTGTTTTGGG	50.0	50.0	58.3	57.9	329-416 bp	1.0	87.9