Hotspot

trnM-CAU-rps14

LSC · 42197-42334 · 138 bp

Back to Vallisneria

Pi 0.172

MarkerSeek score 80.20

Primer available yes

Label trnM-CAU-rps14

Feature Metadata

label_name: trnM-CAU-rps14
feature_type: igs
region: LSC
length_bp: 138
variable_sites: 23
pi: 0.172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-rps14_p1	GAACCCGTGACTTCAAGGTT	GCGCAACAAGATCAAGTTGG	50.0	50.0	58.0	58.3	218-247 bp	1.0	86.5
trnM-CAU-rps14_p2	GAACCCGTGACTTCAAGGTT	GGCGCAACAAGATCAAGTTG	50.0	50.0	58.0	58.3	219-248 bp	1.0	86.5
trnM-CAU-rps14_p3	TTGAACCCGTGACTTCAAGG	GCGCAACAAGATCAAGTTGG	50.0	50.0	58.0	58.3	220-249 bp	1.0	86.4
trnM-CAU-rps14_p4	TTGAACCCGTGACTTCAAGG	GGCGCAACAAGATCAAGTTG	50.0	50.0	58.0	58.3	221-250 bp	1.0	86.4
trnM-CAU-rps14_p5	GGGATTTGAACCCGTGACTT	GCGCAACAAGATCAAGTTGG	50.0	50.0	58.1	58.3	225-254 bp	1.0	85.8