Hotspot

trnL-UAG-trnN-GUU

IRa · 130670-131965 · 1296 bp

Back to Valeriana

Pi 0.159

MarkerSeek score 68.80

Primer available yes

Label trnL-UAG-trnN-GUU

Feature Metadata

label_name: trnL-UAG-trnN-GUU
feature_type: igs
region: IRa
length_bp: 1296
variable_sites: 412
pi: 0.159
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-trnN-GUU_p1	TACCGATTTCACCATAGCGG	CTACCACTGAGCTACTGAGGA	50.0	52.4	57.5	58.0	1172-1565 bp	0.875	74.8
trnL-UAG-trnN-GUU_p2	TACCGATTTCACCATAGCGG	TCTACCACTGAGCTACTGAGG	50.0	52.4	57.5	58.0	1173-1566 bp	0.875	74.8
trnL-UAG-trnN-GUU_p3	TACCGATTTCACCATAGCGG	TACCACTGAGCTACTGAGGA	50.0	50.0	57.5	56.8	1171-1564 bp	0.875	73.3
trnL-UAG-trnN-GUU_p4	TACCGATTTCACCATAGCGG	CGAACGCTCTACCACTGAG	50.0	57.9	57.5	57.7	1180-1573 bp	0.875	72.3
trnL-UAG-trnN-GUU_p5	TACCGATTTCACCATAGCGG	CTACCACTGAGCTACTGAGG	50.0	55.0	57.5	56.5	1172-1565 bp	0.875	70.6