Hotspot

ndhF-ndhD

SSC · 127823-128142 · 320 bp

Back to Valeriana

Pi 0.0722

MarkerSeek score 70.50

Primer available yes

Label ndhF-ndhD

Feature Metadata

label_name: ndhF-ndhD
feature_type: igs
region: SSC
length_bp: 320
variable_sites: 59
pi: 0.0722
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-ndhD_p1	AAGAAGAAGTCCCAGTCCGA	TTTGACCGCTGTACATTGCT	50.0	45.0	58.0	58.1	376-523 bp	0.875	85.9
ndhF-ndhD_p2	AAGAAGAAGTCCCAGTCCGA	GCCTACTACCACCAACCAAG	50.0	55.0	58.0	57.9	852-999 bp	0.875	82.0
ndhF-ndhD_p3	AAGAAGAAGTCCCAGTCCGA	ACTACCACCAACCAAGGAGA	50.0	50.0	58.0	57.9	848-995 bp	0.875	82.0
ndhF-ndhD_p4	AAGAAGAAGTCCCAGTCCGA	ATTCCACCCATTTCGTCGAG	50.0	50.0	58.0	58.0	1100-1247 bp	0.875	81.8
ndhF-ndhD_p5	ACACTTTGAAAGGCCCACAT	ATTCCACCCATTTCGTCGAG	45.0	50.0	57.9	58.0	1150-1297 bp	0.875	81.0