Hotspot

trnN-GUU-trnL-UAG

IRb · 108825-110120 · 1296 bp

Back to Valeriana

Pi 0.216

MarkerSeek score 70.40

Primer available yes

Label trnN-GUU-trnL-UAG

Feature Metadata

label_name: trnN-GUU-trnL-UAG
feature_type: igs
region: IRb
length_bp: 1296
variable_sites: 594
pi: 0.216
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnL-UAG_p1	CTACCACTGAGCTACTGAGGA	AGCGTGTCTACCGATTTCAC	52.4	50.0	58.0	58.0	1180-1346 bp	0.875	78.9
trnN-GUU-trnL-UAG_p2	TCTACCACTGAGCTACTGAGG	AGCGTGTCTACCGATTTCAC	52.4	50.0	58.0	58.0	1181-1347 bp	0.875	78.9
trnN-GUU-trnL-UAG_p3	CTACCACTGAGCTACTGAGGA	CAGCGTGTCTACCGATTTCA	52.4	50.0	58.0	58.0	1181-1347 bp	0.875	78.9
trnN-GUU-trnL-UAG_p4	TCTACCACTGAGCTACTGAGG	CAGCGTGTCTACCGATTTCA	52.4	50.0	58.0	58.0	1182-1348 bp	0.875	78.9
trnN-GUU-trnL-UAG_p5	CTACCACTGAGCTACTGAGGA	TCCTAAGAGCAGCGTGTCTA	52.4	50.0	58.0	57.9	1190-1356 bp	0.875	78.0