Hotspot

infA-rps8

LSC · 79049-79159 · 111 bp

Back to Valeriana

Pi 0.0706

MarkerSeek score 68.70

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 111
variable_sites: 21
pi: 0.0706
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	CACTTGACCCCGTACATCTG	TGGGTCTCACGCATATACCT	55.0	50.0	58.0	57.9	1370-1407 bp	1.0	86.4
infA-rps8_p2	CACTTGACCCCGTACATCTG	GATCAGTCGACCCGGTTTAC	55.0	55.0	58.0	58.1	997-1023 bp	1.0	86.2
infA-rps8_p3	CACTTGACCCCGTACATCTG	GAAGGAACTCGCGTTTTTGG	55.0	50.0	58.0	58.0	1543-1557 bp	0.875	78.5
infA-rps8_p4	GGCAGAAGACCAAGAAACCA	GAAGGAACTCGCGTTTTTGG	50.0	50.0	58.0	58.0	1561-1575 bp	0.875	78.4
infA-rps8_p5	CGAACCCGGAACATACCATT	GAAGGAACTCGCGTTTTTGG	50.0	50.0	58.0	58.0	889-896 bp	0.75	73.9