Hotspot

pafII-cemA

LSC · 59330-59758 · 429 bp

Back to Valeriana

Pi 0.0424

MarkerSeek score 68.70

Primer available yes

Label pafII-cemA

Feature Metadata

label_name: pafII-cemA
feature_type: igs
region: LSC
length_bp: 429
variable_sites: 52
pi: 0.0424
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafII-cemA_p1	AGGGGGCTATTCCTTTGACT	TTCCTATACGCGGGTTTTGG	50.0	50.0	58.0	58.0	793-817 bp	1.0	88.8
pafII-cemA_p2	AGGGGGCTATTCCTTTGACT	ATTCATAGCCGCGGATCATC	50.0	50.0	58.0	58.0	1251-1267 bp	0.625	66.4
pafII-cemA_p3	AGGGGGCTATTCCTTTGACT	GATTCATAGCCGCGGATCAT	50.0	50.0	58.0	58.0	1252-1268 bp	0.625	66.4
pafII-cemA_p4	AACGGGGTCTCGAAAAACAA	ATTCATAGCCGCGGATCATC	45.0	50.0	58.0	58.0	1658-1674 bp	0.625	66.1
pafII-cemA_p5	AACGGGGTCTCGAAAAACAA	GATTCATAGCCGCGGATCAT	45.0	50.0	58.0	58.0	1659-1675 bp	0.625	66.1