Hotspot

trnE-UUC-trnT-GGU

LSC · 30194-31126 · 933 bp

Back to Valeriana

Pi 0.04
MarkerSeek score 78.30
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
933
variable_sites
100
pi
0.04
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AGAGATGTCCTGAACCGCTA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 959-1041 bp 1.0 90.3
trnE-UUC-trnT-GGU_p2 AGATGTCCTGAACCGCTAGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 957-1039 bp 1.0 90.3
trnE-UUC-trnT-GGU_p3 AGAGATGTCCTGAACCGCTA TGGCGTTACTCTACCACTGA 50.0 50.0 57.9 58.1 956-1038 bp 1.0 90.1
trnE-UUC-trnT-GGU_p4 AGATGTCCTGAACCGCTAGA TGGCGTTACTCTACCACTGA 50.0 50.0 57.9 58.1 954-1036 bp 1.0 90.1
trnE-UUC-trnT-GGU_p5 GATGTCCTGAACCGCTAGAC CCATGGCGTTACTCTACCAC 55.0 55.0 57.8 58.1 956-1038 bp 1.0 89.8