Hotspot

atpF-atpH

LSC · 12381-12772 · 392 bp

Back to Valeriana

Pi 0.0449

MarkerSeek score 69.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 392
variable_sites: 47
pi: 0.0449
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	AGAAAGAGTACCATGCTGCG	GCTGTAGAAGGTATCGCGAG	50.0	55.0	58.0	58.0	907-941 bp	1.0	87.2
atpF-atpH_p2	AGAAAGAGTACCATGCTGCG	TGATTTCTGCTGCTTCCGTT	50.0	45.0	58.0	58.1	999-1033 bp	1.0	86.3
atpF-atpH_p3	AGGAAAAAGTACAGCTGGGC	GCTGTAGAAGGTATCGCGAG	50.0	55.0	58.1	58.0	715-749 bp	0.875	82.4
atpF-atpH_p4	CAATACCCCGAGCACTACAC	GCTGTAGAAGGTATCGCGAG	55.0	55.0	58.1	58.0	656-672 bp	0.875	78.7
atpF-atpH_p5	AGAAAGAGTACCATGCTGCG	TTGTGCGTTGCGTTGAATTT	50.0	40.0	58.0	58.1	1319-1342 bp	0.625	67.0