Hotspot

rps11-rpl36

LSC · 88774-88926 · 153 bp

Back to Vachellia

Pi 0.0277

MarkerSeek score 65.70

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 153
variable_sites: 10
pi: 0.0277
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CGTCCATTTCTACGTGAACCA	CGTAGACGCGGACGAATTAT	47.6	50.0	58.3	58.0	257-273 bp	1.0	74.1
rps11-rpl36_p2	CGTCCATTTCTACGTGAACC	CGTAGACGCGGACGAATTAT	50.0	50.0	56.6	58.0	257-273 bp	1.0	73.1
rps11-rpl36_p3	CGTCCATTTCTACGTGAACCA	TCGTAGACGCGGACGAATTA	47.6	50.0	58.3	59.0	258-274 bp	1.0	66.3
rps11-rpl36_p4	CGTCCATTTCTACGTGAACC	TCGTAGACGCGGACGAATTA	50.0	50.0	56.6	59.0	258-274 bp	1.0	65.4
rps11-rpl36_p5	CGTCCATTTCTACGTGAACCA	CTGATTCGTAGACGCGGAC	47.6	57.9	58.3	58.1	263-279 bp	1.0	65.0