Hotspot

ndhC-trnV-UAC

LSC · 58203-59194 · 992 bp

Back to Vachellia

Pi 0.0255
MarkerSeek score 78.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
992
variable_sites
51
pi
0.0255
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.87
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGGCCCTTTATTAATCGGGG AAAAGATGCACGTTGGGTCT 50.0 45.0 57.3 58.0 1113-1217 bp 1.0 82.0
ndhC-trnV-UAC_p2 TGGCCCTTTATTAATCGGGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 1042-1146 bp 1.0 80.8
ndhC-trnV-UAC_p3 TGGCCCTTTATTAATCGGGG GATGCACGTTGGGTCTGTAA 50.0 50.0 57.3 58.2 1109-1213 bp 1.0 80.6
ndhC-trnV-UAC_p4 TGGCCCTTTATTAATCGGGG ATGCACGTTGGGTCTGTAAA 50.0 45.0 57.3 57.7 1108-1212 bp 1.0 80.1
ndhC-trnV-UAC_p5 TGGCCCTTTATTAATCGGGG ACGTTGGGTCTGTAAAGCAA 50.0 45.0 57.3 57.7 1104-1208 bp 1.0 79.5