Hotspot

trnG-GCC-trnfM-CAU

LSC · 43396-43627 · 232 bp

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Pi 0.0216
MarkerSeek score 65.20
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
232
variable_sites
12
pi
0.0216
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 CTTTGCCAAAGAGAAGACGC CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.7 58.0 295-310 bp 1.0 84.3
trnG-GCC-trnfM-CAU_p2 CTTTGCCAAAGAGAAGACGC TGAGGTCACGGGTTCAAATC 50.0 50.0 57.7 57.8 293-308 bp 1.0 83.2
trnG-GCC-trnfM-CAU_p3 CTTTGCCAAAGAGAAGACGC GTAGCTCGCAAGGCTCATAA 50.0 50.0 57.7 57.8 316-331 bp 1.0 82.6
trnG-GCC-trnfM-CAU_p4 CTTTGCCAAAGAGAAGACGC TAGCTCGCAAGGCTCATAAC 50.0 50.0 57.7 57.8 315-330 bp 1.0 82.6
trnG-GCC-trnfM-CAU_p5 CTTTGCCAAAGAGAAGACGC TCTCTTTATCGCGGGGTAGA 50.0 50.0 57.7 57.6 344-359 bp 1.0 81.3