Hotspot

psbZ-trnG-GCC

LSC · 42642-43324 · 683 bp

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Pi 0.0407

MarkerSeek score 75.80

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 683
variable_sites: 44
pi: 0.0407
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	GCGTCTTCTCTTTGGCAAAG	55.0	50.0	58.0	57.7	635-862 bp	1.0	85.7
psbZ-trnG-GCC_p2	TTGCTTCTCCTGATGGTTGG	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	57.8	57.7	638-865 bp	1.0	84.1
psbZ-trnG-GCC_p3	TCCTGATGGTTGGTCGAGTA	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	57.8	57.7	631-858 bp	1.0	84.0
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	GCGTCTTCTCTTTGGCAAAG	55.0	50.0	58.3	57.7	636-863 bp	1.0	83.7
psbZ-trnG-GCC_p5	TTCTCCTGATGGTTGGTCGA	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	58.4	57.7	634-861 bp	1.0	82.9