Hotspot

rps2-rpoC2

LSC · 20346-20589 · 244 bp

Back to Vachellia

Pi 0.0371

MarkerSeek score 75.10

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 244
variable_sites: 20
pi: 0.0371
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CCATGGCCAAAATGAACTCC	TCAGAGCAATCATTTCGAGGA	50.0	42.9	57.3	57.1	329-348 bp	1.0	68.1
rps2-rpoC2_p2	GGCCAAAATGAACTCCTGCT	TCAGAGCAATCATTTCGAGGA	50.0	42.9	58.7	57.1	325-344 bp	1.0	67.7
rps2-rpoC2_p3	TGGCCAAAATGAACTCCTGC	TCAGAGCAATCATTTCGAGGA	50.0	42.9	59.0	57.1	326-345 bp	1.0	65.3
rps2-rpoC2_p4	GCCAAAATGAACTCCTGCTTC	TCAGAGCAATCATTTCGAGGA	47.6	42.9	58.0	57.1	324-343 bp	1.0	65.2
rps2-rpoC2_p5	GGTGCCATTCTAGGATTCCA	TCAGAGCAATCATTTCGAGGA	50.0	42.9	57.0	57.1	359-378 bp	1.0	64.9