Hotspot

trnS-GCU-trnG-UCC

LSC · 9842-11856 · 2015 bp

Back to Vachellia

Pi 0.0314

MarkerSeek score 74.50

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 2015
variable_sites: 125
pi: 0.0314
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	TGAATCAAACGAGGGATCCC	50.0	50.0	58.0	57.3	1886-2127 bp	1.0	83.3
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	TGAATCAAACGAGGGATCCC	50.0	50.0	58.0	57.3	1885-2126 bp	1.0	83.3
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	TGAATCAAACGAGGGATCCC	50.0	50.0	58.2	57.3	1940-2181 bp	1.0	82.0
trnS-GCU-trnG-UCC_p4	TGGAAACGGAAAGAGAGGGA	TGAATCAAACGAGGGATCCC	50.0	50.0	58.3	57.3	1945-2186 bp	1.0	81.0
trnS-GCU-trnG-UCC_p5	CAATCCGACGCTTTAGTCCA	GGATCCATAGAACGAATCGCA	50.0	47.6	58.0	58.0	1854-2095 bp	1.0	80.9