Hotspot

trnQ-UUG-psbK

LSC · 7821-8695 · 875 bp

Back to Vachellia

Pi 0.0183

MarkerSeek score 68.00

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 875
variable_sites: 38
pi: 0.0183
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GATATGCTCTGGGACGGAAG	AGGCTTCAGGTAATTTCGCG	55.0	50.0	57.6	58.6	1043-1187 bp	1.0	78.0
trnQ-UUG-psbK_p2	GATATGCTCTGGGACGGAAG	GGCTTCAGGTAATTTCGCGA	55.0	50.0	57.6	58.6	1042-1186 bp	1.0	78.0
trnQ-UUG-psbK_p3	GACCAAAACCCGTTGCCTTA	AGGCTTCAGGTAATTTCGCG	50.0	50.0	58.7	58.6	1001-1145 bp	1.0	76.4
trnQ-UUG-psbK_p4	GACCAAAACCCGTTGCCTTA	GGCTTCAGGTAATTTCGCGA	50.0	50.0	58.7	58.6	1000-1144 bp	1.0	76.4
trnQ-UUG-psbK_p5	AATGGATATGCTCTGGGACG	AGGCTTCAGGTAATTTCGCG	50.0	50.0	57.2	58.6	1047-1191 bp	1.0	74.8