Hotspot

ndhG-ndhI

Genome · 148563-151109 · 2547 bp

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Pi 0.104

MarkerSeek score 75.20

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: Genome
length_bp: 2547
variable_sites: 537
pi: 0.104
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.52
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AAGACCCAATCCCAGGAAGA	AGGCAATGGAATAAAGCATCA	50.0	38.1	57.9	55.9	1408-2643 bp	0.714286	49.6
ndhG-ndhI_p2	AAGACCCAATCCCAGGAAGA	CGTCTCATCATAAAAGGCAATGG	50.0	43.5	57.9	58.1	1422-2657 bp	0.714286	49.3
ndhG-ndhI_p3	AAGACCCAATCCCAGGAAGA	GGCAATGGAATAAAGCATCAAGA	50.0	39.1	57.9	57.7	1407-2642 bp	0.714286	48.4
ndhG-ndhI_p4	AAGACCCAATCCCAGGAAGA	AGGCAATGGAATAAAGCATCAAG	50.0	39.1	57.9	57.7	1408-2643 bp	0.714286	48.4
ndhG-ndhI_p5	AAGACCCAATCCCAGGAAGA	AGGCAATGGAATAAAGCATCAA	50.0	36.4	57.9	56.6	1408-2643 bp	0.714286	47.6