Hotspot

rpl32-ndhF

Genome · 138854-140254 · 1401 bp

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Pi 0.0381

MarkerSeek score 75.30

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: Genome
length_bp: 1401
variable_sites: 132
pi: 0.0381
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	AGCGTGTCTACCGATTTCAC	GGGAATTGCGCAGATTTAGC	50.0	50.0	58.0	57.9	1839-2866 bp	0.857143	82.2
rpl32-ndhF_p2	AGCGTGTCTACCGATTTCAC	TACGTGAAGAGGGAATTGCG	50.0	50.0	58.0	58.0	1849-2876 bp	0.714286	75.0
rpl32-ndhF_p3	CAGCGTGTCTACCGATTTCA	TACGTGAAGAGGGAATTGCG	50.0	50.0	58.0	58.0	1850-2877 bp	0.714286	75.0
rpl32-ndhF_p4	TCCTAAGAGCAGCGTGTCTA	TACGTGAAGAGGGAATTGCG	50.0	50.0	57.9	58.0	1859-2886 bp	0.714286	74.1
rpl32-ndhF_p5	AGCTTTTAAGGCTGCCCAAT	TACGTGAAGAGGGAATTGCG	45.0	50.0	58.1	58.0	2215-2238 bp	0.571429	65.9