Hotspot

ndhI-ndhG

Genome · 131036-133582 · 2547 bp

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Pi 0.0997

MarkerSeek score 75.20

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: Genome
length_bp: 2547
variable_sites: 521
pi: 0.0997
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.52
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	AGGCAATGGAATAAAGCATCA	AAGACCCAATCCCAGGAAGA	38.1	50.0	55.9	57.9	1408-2643 bp	0.714286	49.6
ndhI-ndhG_p2	CGTCTCATCATAAAAGGCAATGG	AAGACCCAATCCCAGGAAGA	43.5	50.0	58.1	57.9	1422-2657 bp	0.714286	49.3
ndhI-ndhG_p3	GGCAATGGAATAAAGCATCAAGA	AAGACCCAATCCCAGGAAGA	39.1	50.0	57.7	57.9	1407-2642 bp	0.714286	48.4
ndhI-ndhG_p4	AGGCAATGGAATAAAGCATCAAG	AAGACCCAATCCCAGGAAGA	39.1	50.0	57.7	57.9	1408-2643 bp	0.714286	48.4
ndhI-ndhG_p5	AGGCAATGGAATAAAGCATCAA	AAGACCCAATCCCAGGAAGA	36.4	50.0	56.6	57.9	1408-2643 bp	0.714286	47.6