Hotspot

trnR-ACG-trnN-GUU

Genome · 120087-120599 · 513 bp

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Pi 0.147

MarkerSeek score 76.70

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: Genome
length_bp: 513
variable_sites: 256
pi: 0.147
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CTCGACGCCAGGATGTTAAA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	58.0	58.0	924-928 bp	1.0	91.9
trnR-ACG-trnN-GUU_p2	CTCGACGCCAGGATGTTAAA	CAGTACCCTCGTTGACTTCG	50.0	55.0	58.0	58.0	1221-1225 bp	1.0	91.8
trnR-ACG-trnN-GUU_p3	CCTTGTTCCTACATGACCCG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	58.0	58.0	1271-1275 bp	1.0	91.8
trnR-ACG-trnN-GUU_p4	CCTTGTTCCTACATGACCCG	CAGTACCCTCGTTGACTTCG	55.0	55.0	58.0	58.0	1568-1572 bp	1.0	91.6
trnR-ACG-trnN-GUU_p5	TGCCTCTCCAAGGGATGTAA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	58.0	58.0	1149-1151 bp	0.285714	51.3